7-AAD Solution

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Overview

Cell viability assay for assessing cell health during flow cytometry. It can be added directly to samples to bypass traditional manual weighing, pipetting, and dilution steps.

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Description

Description

The ReadiDrop™ 7-aminoactinomycin D (7-AAD) cell viability assay is designed as a ready-to-use 7-AAD solution, which binds double-stranded DNA with a high-affinity intercalation between GC base pairs.

The assay removes the traditional manual weighing, pipetting, and dilution steps when using commonly available cell viability dyes in flow cytometry experiments. Simply add 1 or 2 drops of the cell viability assay to assess the health of your cells.

Key Benefits of the ReadiDrop 7-AAD Cell Viability Dye

  • Ideal for cell sorting — fluorescent molecules are suspended in PBS without preservatives
  • Stable at room temperature — keep a bottle of ReadiDrop 7-AAD cell viability assay by your cell sorter or cell analyzer for easy access
  • No more weighing, pipetting, and diluting — ready-to-use solution gives 1 µg/ml as the final concentration

Features of the ReadiDrop 7-AAD Cell Viability Dye

  • Fluorophore Properties
    • Excitation maximum: 548 nm
    • Emission maximum: 648 nm
    • Optimal excitation laser: 561 nm or 488 nm
  • Fluorescent Spectrum

 Fluorescent Spectrum

  • Molecular weight: 1270.43 g/mol
  • Molecular formula: C27H34I2N4
  • Chemical structure

Chemical Structure

  • Specifications
    • Store at 15–30°C
    • Do not freeze

More Information

Learn more about the features of the S3e™ Cell Sorter and its associated consumables.

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(Discontinued) ReadiDrop™ 7-AAD
1351102
9 ml, (3 x 3 ml) ready-to-use dropper bottles for dead cell exclusion

Documents

Documents

Number Description Options
6522
ReadiDrop Cell Viability Assays Flier, Rev B
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10049240
ReadiDrop™ Cell Viability Assays Product Insert, Rev A