Gene:  MME, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype which represent 85% of cases of ALL. This protein is not restricted to leukemic cells however and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon enkephalins substance P neurotensin oxytocin and bradykinin. This gene which encodes a 100-kD type II transmembrane glycoprotein exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: MME, Human
membrane metallo-endopeptidase

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0005367
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PrimePCR™ SYBR® Green Assay: MME, Human
membrane metallo-endopeptidase

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0048099
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055359
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PrimePCR™ Probe Assay: MME, Human
membrane metallo-endopeptidase

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0055359
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PrimePCR™ ddPCR™ Copy Number Assay: MME, Human
membrane metallo-endopeptidase

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000176
Info:   74nt amplicon; intronic
 
Fluorophore:   FAM
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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