PrimePCR™ SYBR® Green Assay: MME, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055359

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048099
Assay Design:   exonic
Chromosome Location:   3:154801987-154802096question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000492661 ENST00000460393 ENST00000481828 ENST00000382989 ENST00000462745 ENST00000493237 ENST00000360490 ENST00000491026 ENST00000473730 ENST00000462837

Gene Information

This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype which represent 85% of cases of ALL. This protein is not restricted to leukemic cells however and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon enkephalins substance P neurotensin oxytocin and bradykinin. This gene which encodes a 100-kD type II transmembrane glycoprotein exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq Jul 2008]

Gene Symbol:   MME
Gene Name:   membrane metallo-endopeptidase
Aliases:   CALLA, CD10, DKFZp686O16152, MGC126681, MGC126707, NEP, SFE
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000196549
Entrez:   4311
UniGene:   Hs.307734
Chromosome Mapping:   3q25.1-q25.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.070000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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