This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: MME, Human
PrimePCR™ Template for SYBR® Green Assay: MME, Human
This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype which represent 85% of cases of ALL. This protein is not restricted to leukemic cells however and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon enkephalins substance P neurotensin oxytocin and bradykinin. This gene which encodes a 100-kD type II transmembrane glycoprotein exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.