Description
Description
The PrecisionAb Antibody product line is a collection of highly specific and sensitive primary antibodies (monoclonal and polyclonal) that have been extensively validated for western blotting.
Features and Benefits of PrecisionAb Antibodies
- Robust validation — performed on up to 12 different cell lysates with endogenous protein levels
- Reproducible data — stringent batch-to-batch QC
- High specificity and sensitivity — guarantee low nonspecific binding and strong signal from target proteins
Learn about our rigorous
validation process
Download the poster and watch the video of Dr. Mark Shulewitz as he walks you through the poster which discusses the validation steps for our premium line of PrecisonAb Primary Antibodies.
Watch Video | Download Poster
User Validation
Side-by-side testing of anti-human Bcl-2 antibodies with Jurkat call lysate
“Antibodies provided from Bio-Rad were of very high quality for 80% of the antibodies tested, suggesting most antibodies will be great for western blotting. The other antibody tested was of high quality, on par with the best antibodies available for the target protein.”
Prof. Aldrin V. Gomes, University of California, Davis
Comparing Bcl-2 levels in 12 human cell lines using the PrecisionAb BCL-2-specific antibody
“For our biochemical experiments, we select high-performance reagents that give us the best signal-to-noise ratio. Bio-Rad antibodies against Bcl-2 are high-performance antibodies, as indicated by single, clean, and clear bands, with no noticeable background signal at a high dilution of 1:1,000.”
Prof. Ernesto Diaz-Flores, University of California, San Francisco
Citations
PrecisionAb Antibodies have helped identify key proteins on western blots in the following published work:
- Serban AI et al. (2016). RAGE and TGF-β1 cross-talk regulate extracellular matrix turnover and cytokine synthesis in AGEs exposed fibroblast cells. PLOS ONE. PMCID: PMC4807770
- Serban AI et al. (2015). AGEs-Induced IL-6 synthesis precedes RAGE up-regulation in HEK 293 cells: An alternative inflammatory mechanism? Int J Mol Sci 16, 20100–20117. PMCID: PMC4613191
More Information
PrecisionAb Antibody Development
Learn how Bio-Rad researchers have defined new quality standards for superior results and reproducibility in western blotting antibodies.
Download PrecisionAb Antibodies flier for:
Western Blotting
Cancer Research
Neuroscience Research
Related Articles
Reliable Antibodies for Reproducible Western Blots: New Line of Antibodies Raises Industry Standards for Quality
Genetic Engineering News, January 2016
How Does an Antibody Qualify to Become a PrecisionAb Antibody?
Bioradiations, March 8, 2016
How Can Antibodies Ensure Your Research Stands the Test of Time?
Bioscience Technology, January 2016
Is There an Antibody Crisis Looming?
Laboratory News May 24, 2016
PrecisionAb Antibodies: Defining New Standards for Western Blotting
Bioradiations, February 9, 2016
High-Throughput Validation of Antibodies for Cancer Research
AACR Poster, 2016
Validating Antibodies – the Good, the Bad, and the Necessary
DDNews, February 2017
Technical Resources
Experimental Protocol for Validating PrecisionAb Antibodies Using the Stain-Free Western Blotting Workflow
This protocol describes our validation process and gives you, the user, an opportunity to verify our results yourself
Instructional Protocol for PrecisionAb Antibodies Customers
This protocol describes how to use PrecisionAb Antibodies to get the best western blotting results
Western Blotting Doctor
Comprehensive solutions and suggestions to help solve your particular western blotting challenges