ddPCR Copy Number Determination Assays

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Bio-Rad’s ddPCR Copy Number Determination Assays offer high precision and resolution to discriminate between small changes in copy number.

Digital PCR can easily detect small fold changes such as 1.2 fold change in CNV from 5 to 6 copies. A major technical challenge in copy number assessment is the ability to discriminate, with statistical confidence, between consecutive copy number states. Partitioning of the ddPCR reaction enables the discrimination required to resolve small fold changes. Browse list of wet-lab validated Copy Number Determination Assays and Centromeric CNV Assays.

Key Benefits

• Wet-lab validated assays available for more than 700 targets
• Centromeric reference assays available for more than 60 targets
• Wet-lab validated assays guaranteed to work when used as instructed
• Validation data available for review and download
• Assays are designed instantly for targets and sequences for which validated assays are not available
• Assays orderable in multiple probe fluorophores
• Reference assays available

Sample Data

PrimePCR ddPCR copy number assays provide superior resolution and precision. A, 2-D fluorescence amplitude plot shows four replicate wells of a copy number sample duplexed with ERBB2 and RPP30 assays. The black cluster on the plot represents the negative droplets, the green cluster represents the droplets that are positive for RPP30 reference only, the blue cluster represents the droplets that are positive for ERBB2 only, and the orange cluster represents the droplets that are positive for both ERBB2 and RPP30 targets. B, copy number plot shows multiple wells at copy number 2 with precise replicate and merged well values. C, copy number plot shows multiple oncogene copy number determinations for various genes with the same NCI-H358 lung cancer sample. All error bars are generated by QuantaSoft software and represent the 95% confidence interval.

Packaging Options

ddPCR Copy Number Determination Assays are available in multiple probe fluorophores. The ddPCR CNV Assays are provided in a 20X, ready-to-use primer-probe mix optimized for use with ddPCR Supermix for Probes (No dUTP). Each tube contains 18uM primers and 5uM probe

Related Products

• ddPCR Supermix for Probes no dUTP
• ddPCR Supermix for Probes
• ddPCR SMN1 Copy Number Determination Kit
• ddPCR SMN2 Copy Number Determination Kit