Gene:  WAS, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli and interact with multiple proteins. Recent studies have demonstrated that these proteins directly or indirectly associate with the small GTPase Cdc42 known to regulate formation of actin filaments and the cytoskeletal organizing complex Arp2/3. Wiskott-Aldrich syndrome is a rare inherited X-linked recessive disease characterized by immune dysregulation and microthrombocytopenia and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein expressed exclusively in hematopoietic cells which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage and containing a different 5' UTR sequence has been described however its full-length nature is not known. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: WAS, Human
Wiskott-Aldrich syndrome (eczema-thrombocytopenia)

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0004639
Info:   Same primer pair as used in probe assay qHsaCEP0025099
List Price:    $125.00
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PrimePCR™ Probe Assay: WAS, Human
Wiskott-Aldrich syndrome (eczema-thrombocytopenia)

Assay Type: Probe
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0025099
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: WAS, Human
Wiskott-Aldrich syndrome (eczema-thrombocytopenia)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5002816
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0004639; Exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: WAS, Human
Wiskott-Aldrich syndrome (eczema-thrombocytopenia)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5027092
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0025099; Exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: WAS, Human
Wiskott-Aldrich syndrome (eczema-thrombocytopenia)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5027093
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0025099; Exonic
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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