PrimePCR™ SYBR® Green Assay: WAS, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0025099

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004639
Assay Design:   Exonic
Chromosome Location:   X:48546689-48546812question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000376701

Gene Information

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli and interact with multiple proteins. Recent studies have demonstrated that these proteins directly or indirectly associate with the small GTPase Cdc42 known to regulate formation of actin filaments and the cytoskeletal organizing complex Arp2/3. Wiskott-Aldrich syndrome is a rare inherited X-linked recessive disease characterized by immune dysregulation and microthrombocytopenia and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein expressed exclusively in hematopoietic cells which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage and containing a different 5' UTR sequence has been described however its full-length nature is not known. [provided by RefSeq Jul 2008]

Gene Symbol:   WAS
Gene Name:   Wiskott-Aldrich syndrome (eczema-thrombocytopenia)
Aliases:   IMD2, THC, THC1, WASP
RefSeq:   NC_000023.10 NG_007877.1 NT_079573.4
Ensembl:   ENSG00000015285
Entrez:   7454
UniGene:   Hs.2157
Chromosome Mapping:   Xp11.4-p11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.810000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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