This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0025099
PrimePCR™ PreAmp for SYBR® Green Assay: WAS, Human
PrimePCR™ Template for SYBR® Green Assay: WAS, Human
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli and interact with multiple proteins. Recent studies have demonstrated that these proteins directly or indirectly associate with the small GTPase Cdc42 known to regulate formation of actin filaments and the cytoskeletal organizing complex Arp2/3. Wiskott-Aldrich syndrome is a rare inherited X-linked recessive disease characterized by immune dysregulation and microthrombocytopenia and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein expressed exclusively in hematopoietic cells which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage and containing a different 5' UTR sequence has been described however its full-length nature is not known. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.