Gene:  PTCH1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus syndrome esophageal squamous cell carcinoma trichoepitheliomas transitional cell carcinomas of the bladder as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described but their full length sequences and biological validity cannot be determined currently. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: PTCH1, Human
patched 1

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0001809
Info:   Recommended - best coverage
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PrimePCR™ SYBR® Green Assay: PTCH1, Human
patched 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0047782
Info:   Same primer pair as used in probe assay qHsaCEP0055042
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PrimePCR™ Probe Assay: PTCH1, Human
patched 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0055042
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PrimePCR™ ddPCR™ Copy Number Assay: PTCH1, Human
patched 1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000505
Info:   68nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PTCH1 p.R602*, Human
patched 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500730
Info:   70nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PTCH1 WT for p.R602*, Human
patched 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500731
Info:   70nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PTCH1 p.Q816*, Human
patched 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500776
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PTCH1 WT for p.Q816*, Human
patched 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500777
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PTCH1 p.T807A, Human
patched 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501734
Info:   77nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PTCH1 WT for p.T807A, Human
patched 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501735
Info:   77nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PTCH1 p.Q365*, Human
patched 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502566
Info:   96nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PTCH1 WT for p.Q365*, Human
patched 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502567
Info:   96nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PTCH1 p.W1018*, Human
patched 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502842
Info:   59nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PTCH1 WT for p.W1018*, Human
patched 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502843
Info:   59nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PTCH1 p.M561R, Human
patched 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503432
Info:   62nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PTCH1 WT for p.M561R, Human
patched 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503433
Info:   62nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PTCH1 p.W236*, Human
patched 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504104
Info:   66nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PTCH1 WT for p.W236*, Human
patched 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504105
Info:   66nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PTCH1 p.R135*, Human
patched 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504620
Info:   79nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PTCH1 WT for p.R135*, Human
patched 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504621
Info:   79nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PTCH1 p.G288G, Human
patched 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504966
Info:   78nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PTCH1 WT for p.G288G, Human
patched 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504967
Info:   78nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PTCH1 p.L175P, Human
patched 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504996
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PTCH1 WT for p.L175P, Human
patched 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504997
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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