PrimePCR™ Probe Assay: PTCH1, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055042
Assay Design:   exonic
Chromosome Location:   9:98268712-98268856question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000331920 ENST00000430669 ENST00000429896 ENST00000375274 ENST00000548420 ENST00000553011 ENST00000551845 ENST00000547672 ENST00000546820 ENST00000468211 ENST00000551630 ENST00000437951 ENST00000421141 ENST00000418258

Gene Information

This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus syndrome esophageal squamous cell carcinoma trichoepitheliomas transitional cell carcinomas of the bladder as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described but their full length sequences and biological validity cannot be determined currently. [provided by RefSeq Jul 2008]

Gene Symbol:   PTCH1
Gene Name:   patched 1
Aliases:   BCNS, FLJ26746, FLJ42602, HPE7, NBCCS, PTC, PTC1, PTCH, PTCH11
RefSeq:   NC_000009.11 NG_007664.1 NT_008470.19
Ensembl:   ENSG00000185920
Entrez:   5727
UniGene:   Hs.494538
Chromosome Mapping:   9q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.690000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download