Gene:  PIK3R1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85 55 or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq Jun 2011]

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PrimePCR™ SYBR® Green Assay: PIK3R1, Human
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0045279
Info:   Same primer pair as used in probe assay qHsaCEP0052540
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PrimePCR™ SYBR® Green Assay: PIK3R1, Human
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0023749
Info:   Recommended - best efficiency
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PrimePCR™ Probe Assay: PIK3R1, Human
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0052540
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PrimePCR™ ddPCR™ Copy Number Assay: PIK3R1, Human
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000119
Info:   68nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PIK3R1 p.R348*, Human
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501068
Info:   69nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PIK3R1 WT for p.R348*, Human
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501069
Info:   69nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PIK3R1 p.D464G, Human
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501970
Info:   80nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PIK3R1 WT for p.D464G, Human
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501971
Info:   80nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PIK3R1 p.D560G, Human
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503442
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PIK3R1 WT for p.D560G, Human
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503443
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PIK3R1 p.R649Q, Human
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503876
Info:   78nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PIK3R1 WT for p.R649Q, Human
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503877
Info:   78nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PIK3R1 p.R461*, Human
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504074
Info:   98nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PIK3R1 WT for p.R461*, Human
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504075
Info:   98nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PIK3R1 p.N564D, Human
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505272
Info:   67nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PIK3R1 WT for p.N564D, Human
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505273
Info:   67nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PIK3R1 p.G376R, Human
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2506028
Info:   100nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PIK3R1 WT for p.G376R, Human
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2506029
Info:   100nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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