Gene:  PGK1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 13-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq Oct 2008]

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PrimePCR™ SYBR® Green Assay: PGK1, Human
phosphoglycerate kinase 1

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0003721
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PrimePCR™ SYBR® Green Assay: PGK1, Human
phosphoglycerate kinase 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042912
Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0050174
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PrimePCR™ Probe Assay: PGK1, Human
phosphoglycerate kinase 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050174
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: PGK1, Human
phosphoglycerate kinase 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5007748
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0042912; exonic
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PrimePCR™ ddPCR™ Expression Probe Assay: PGK1, Human
phosphoglycerate kinase 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5033808
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0050174; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: PGK1, Human
phosphoglycerate kinase 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5033809
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0050174; exonic
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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