PrimePCR™ Probe Assay: PGK1, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050174
Assay Design:   exonic
Chromosome Location:   X:77380922-77381435question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000373316 ENST00000442431 ENST00000537456 ENST00000597340 ENST00000593375 ENST00000593893

Gene Information

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 13-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq Oct 2008]

Gene Symbol:   PGK1
Gene Name:   phosphoglycerate kinase 1
Aliases:   MGC117307, MGC142128, MGC8947, MIG10, PGKA
RefSeq:   NC_000023.10 NG_008862.1 NT_011651.17
Ensembl:   ENSG00000102144
Entrez:   5230
UniGene:   Hs.78771
Chromosome Mapping   Xq13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.830000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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