This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: PGK1, Human
PrimePCR™ Template for Probe Assay: PGK1, Human
The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 13-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq Oct 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.
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Aurum™ Total RNA Mini KitThe Aurum total RNA mini kit produces high-quality DNA-free total RNA from a wide range of starting materials including cultured cells, bacteria, and yeast, as well as animal and plant tissues.
iScript™ Advanced cDNA Synthesis Kit for RT-qPCRThe iScript advanced cDNA synthesis kit for RT-qPCR is a simple, fast, and sensitive first-strand cDNA synthesis kit for gene expression analysis by reverse-transcription qPCR.
PrimePCR™ Assays and PanelsExperimentally validated real-time PCR assays are available as individual assays, preplated pathway- and disease-specific panels, or custom- configured plates.
SsoAdvanced™ Universal Real-Time PCR SupermixThe SsoAdvanced™ universal real-time PCR supermixes employ our patented Sso7d fusion enzyme technology for higher processivity, increased PCR inhibitor tolerance, and robust performance with difficult qPCR targets. Available for probe- and dye-based assays in ROX- dependent or ROX-independent real-time PCR detection systems.
Real-Time PCR Detection SystemsReal-time PCR amplification systems combine thermal cyclers with optical reaction modules for singleplex and multiplex detection of fluorophores. All systems feature thermal gradient functionality.
Amplification SoftwareSoftware for Bio-Rad thermal cyclers supports multi-instrument control and collection and analysis of real-time data. Data analysis tools include ΔCq and ΔΔCq analyses and scatter plot, volcano plot, heat map, and clustergram outputs.
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View the products required to complete your real-time PCR experiments.
Pricing for PrimePCR real-time PCR assays is based on assay type, the number of reactions, and plate configuration for 96- and 384-well plates.
Discount applies to bulk orders of the same catalog number and is taken off the list price.
Bulk discounts are shown below; download the pricing flyer for full details.
Real-Time PCR Assays and Controls
5 or more assays
Pathway and Collection Panels
20 or more plates
Custom PlatesCustom 96-well & 384-well plates with 1–96 unique genes
Custom 384-well plates with 97 or more unique genes
30 or more plates
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