Gene:  MBD5, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the methyl-CpG-binding domain (MBD) family. The MBD consists of about 70 residues and is the minimal region required for a methyl-CpG-binding protein binding specifically to methylated DNA. In addition to the MBD domain this protein contains a PWWP domain (Pro-Trp-Trp-Pro motif) which consists of 100-150 amino acids and is found in numerous proteins that are involved in cell division growth and differentiation. Mutations in this gene cause mental retardation autosomal dominant type 1. Haploinsufficiency of this gene is associated with a syndrome involving microcephaly intellectual disabilities severe speech impairment and seizures. Alternatively spliced transcript variants have been found but their full-length nature is not determined. [provided by RefSeq Mar 2010]

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PrimePCR™ SYBR® Green Assay: MBD5, Human
methyl-CpG binding domain protein 5

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0014730
Info:   Same primer pair as used in probe assay qHsaCIP0029530
List Price:    $125.00
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PrimePCR™ Probe Assay: MBD5, Human
methyl-CpG binding domain protein 5

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0029530
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MBD5, Human
methyl-CpG binding domain protein 5

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5020276
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0014730; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: MBD5, Human
methyl-CpG binding domain protein 5

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5052498
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0029530; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: MBD5, Human
methyl-CpG binding domain protein 5

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5052499
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0029530; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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