PrimePCR™ SYBR® Green Assay: MBD5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029530

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014730
Assay Design:   Intron-spanning
Chromosome Location:   2:149227923-149240715question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000407073 ENST00000404807 ENST00000416015

Gene Information

This gene encodes a member of the methyl-CpG-binding domain (MBD) family. The MBD consists of about 70 residues and is the minimal region required for a methyl-CpG-binding protein binding specifically to methylated DNA. In addition to the MBD domain this protein contains a PWWP domain (Pro-Trp-Trp-Pro motif) which consists of 100-150 amino acids and is found in numerous proteins that are involved in cell division growth and differentiation. Mutations in this gene cause mental retardation autosomal dominant type 1. Haploinsufficiency of this gene is associated with a syndrome involving microcephaly intellectual disabilities severe speech impairment and seizures. Alternatively spliced transcript variants have been found but their full-length nature is not determined. [provided by RefSeq Mar 2010]

Gene Symbol:   MBD5
Gene Name:   methyl-CpG binding domain protein 5
Aliases:   FLJ11113, FLJ30517, KIAA1461, MRD1
RefSeq:   NC_000002.11 NG_017003.1 NT_022135.16
Ensembl:   ENSG00000204406
Entrez:   55777
UniGene:   Hs.458312
Chromosome Mapping:   2q23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.830000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download