Gene:  MAPK1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a member of the MAP kinase family. MAP kinases also known as extracellular signal-regulated kinases (ERKs) act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation differentiation transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation this kinase translocates to the nucleus of the stimulated cells where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein but differing in the UTRs have been reported for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: MAPK1, Human
mitogen-activated protein kinase 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042738
Info:   Same primer pair as used in probe assay qHsaCEP0050000
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PrimePCR™ SYBR® Green Assay: MAPK1, Human
mitogen-activated protein kinase 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0006818
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: MAPK1, Human
mitogen-activated protein kinase 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050000
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MAPK1, Human
mitogen-activated protein kinase 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5007588
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0042738; exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MAPK1, Human
mitogen-activated protein kinase 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5014745
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0006818; Intron-spanning
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PrimePCR™ ddPCR™ Expression Probe Assay: MAPK1, Human
mitogen-activated protein kinase 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5033488
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0050000; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: MAPK1, Human
mitogen-activated protein kinase 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5033489
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0050000; exonic
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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