PrimePCR™ SYBR® Green Assay: MAPK1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0006818
Assay Design:   Intron-spanning
Chromosome Location:   22:22160281-22161998question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000215832 ENST00000398822 ENST00000544786 ENST00000415911

Gene Information

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases also known as extracellular signal-regulated kinases (ERKs) act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation differentiation transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation this kinase translocates to the nucleus of the stimulated cells where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein but differing in the UTRs have been reported for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   MAPK1
Gene Name:   mitogen-activated protein kinase 1
Aliases:   ERK, ERK2, ERT1, MAPK2, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk
RefSeq:   NC_000022.10 NG_023054.1 NT_011520.12
Ensembl:   ENSG00000100030
Entrez:   5594
UniGene:   Hs.431850
Chromosome Mapping:   22q11.2,22q11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.080000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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