Gene:  MAP4K4, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1 MAP2K4/MKK4 and MAP2K7/MKK7 which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: MAP4K4, Human
mitogen-activated protein kinase kinase kinase kinase 4

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0009017
Info:   Same primer pair as used in probe assay qHsaCIP0027019
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PrimePCR™ Probe Assay: MAP4K4, Human
mitogen-activated protein kinase kinase kinase kinase 4

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0027019
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PrimePCR™ ddPCR™ Mutation Assay:MAP4K4 p.S259L, Human
mitogen-activated protein kinase kinase kinase kinase 4

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503020
Info:   79nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:MAP4K4 WT for p.S259L, Human
mitogen-activated protein kinase kinase kinase kinase 4

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503021
Info:   79nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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