PrimePCR™ SYBR® Green Assay: MAP4K4, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027019

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009017
Assay Design:   Intron-spanning
Chromosome Location:   2:102490609-102493530question
Amplicon Length:   142
Splice Variants Targeted:   ENST00000425019 ENST00000413150 ENST00000456652 ENST00000347699 ENST00000417294 ENST00000421882 ENST00000324219 ENST00000350198 ENST00000302217 ENST00000350878

Gene Information

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1 MAP2K4/MKK4 and MAP2K7/MKK7 which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   MAP4K4
Gene Name:   mitogen-activated protein kinase kinase kinase kinase 4
Aliases:   FLH21957, FLJ10410, FLJ20373, FLJ90111, HGK, KIAA0687, NIK
RefSeq:   NC_000002.11 NT_022171.15
Ensembl:   ENSG00000071054
Entrez:   9448
UniGene:   Hs.730600
Chromosome Mapping:   2q11.2-q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.230000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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