Gene:  LIMS1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is an adaptor protein which contains five LIM domains or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2 which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2010]

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PrimePCR™ SYBR® Green Assay: LIMS1, Human
LIM and senescent cell antigen-like domains 1

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0001328
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PrimePCR™ SYBR® Green Assay: LIMS1, Human
LIM and senescent cell antigen-like domains 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0046659
Info:   Same primer pair as used in probe assay qHsaCEP0053920
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PrimePCR™ Probe Assay: LIMS1, Human
LIM and senescent cell antigen-like domains 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0053920
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: LIMS1, Human
LIM and senescent cell antigen-like domains 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5000274
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0001328; Exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: LIMS1, Human
LIM and senescent cell antigen-like domains 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5011079
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0046659; exonic
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PrimePCR™ ddPCR™ Expression Probe Assay: LIMS1, Human
LIM and senescent cell antigen-like domains 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5040468
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0053920; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: LIMS1, Human
LIM and senescent cell antigen-like domains 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5040469
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0053920; exonic
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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