PrimePCR™ Probe Assay: LIMS1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LIMS1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LIMS1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053920
Assay Design:   exonic
Chromosome Location:   2:109300343-109300442question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000332345 ENST00000393310 ENST00000410093 ENST00000409441 ENST00000338045 ENST00000544547 ENST00000542845 ENST00000426981 ENST00000409286 ENST00000409754 ENST00000324938 ENST00000409455 ENST00000355119 ENST00000409808 ENST00000410011 ENST00000410038 ENST00000545738 ENST00000409254

Gene Information

The protein encoded by this gene is an adaptor protein which contains five LIM domains or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2 which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2010]

Gene Symbol:   LIMS1
Gene Name:   LIM and senescent cell antigen-like domains 1
Aliases:   PINCH, PINCH1
RefSeq:   NC_000002.11 NT_022171.15
Ensembl:   ENSG00000169756
Entrez:   3987
Chromosome Mapping:   2q12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 37.000000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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