Gene:  LAMB2, Human

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Laminins a family of extracellular matrix glycoproteins are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion differentiation migration signaling neurite outgrowth and metastasis. Laminins composed of 3 non identical chains: laminin alpha beta and gamma (formerly A B1 and B2 respectively) form a cruciform structure consisting of 3 short arms each formed by a different chain and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown but some of the chains have been shown to differ with respect to their tissue distribution presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin beta 2. The beta 2 chain contains the 7 structural domains typical of beta chains of laminin including the short alpha region. However unlike beta 1 chain beta 2 has a more restricted tissue distribution. It is enriched in the basement membrane of muscles at the neuromuscular junctions kidney glomerulus and vascular smooth muscle. Transgenic mice in which the beta 2 chain gene was inactivated by homologous recombination showed defects in the maturation of neuromuscular junctions and impairment of glomerular filtration. Alternative splicing involving a non consensus 5' splice site (gc) in the 5' UTR of this gene has been reported. It was suggested that inefficient splicing of this first intron which does not change the protein sequence results in a greater abundance of the unspliced form of the transcript than the spliced form. The full-length nature of the spliced transcript is not known. [provided by RefSeq Aug 2011]

PrimePCR™ SYBR® Green Assay: LAMB2, Human
laminin, beta 2 (laminin S)

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0001080
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PrimePCR™ SYBR® Green Assay: LAMB2, Human
laminin, beta 2 (laminin S)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0056976
Info:   Same primer pair as used in probe assay qHsaCEP0058081
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PrimePCR™ Probe Assay: LAMB2, Human
laminin, beta 2 (laminin S)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0058081
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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