PrimePCR™ SYBR® Green Assay: LAMB2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0058081

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056976
Assay Design:   exonic
Chromosome Location:   3:49168471-49168826question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000494831 ENST00000305544 ENST00000418109

Gene Information

Laminins a family of extracellular matrix glycoproteins are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion differentiation migration signaling neurite outgrowth and metastasis. Laminins composed of 3 non identical chains: laminin alpha beta and gamma (formerly A B1 and B2 respectively) form a cruciform structure consisting of 3 short arms each formed by a different chain and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown but some of the chains have been shown to differ with respect to their tissue distribution presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin beta 2. The beta 2 chain contains the 7 structural domains typical of beta chains of laminin including the short alpha region. However unlike beta 1 chain beta 2 has a more restricted tissue distribution. It is enriched in the basement membrane of muscles at the neuromuscular junctions kidney glomerulus and vascular smooth muscle. Transgenic mice in which the beta 2 chain gene was inactivated by homologous recombination showed defects in the maturation of neuromuscular junctions and impairment of glomerular filtration. Alternative splicing involving a non consensus 5' splice site (gc) in the 5' UTR of this gene has been reported. It was suggested that inefficient splicing of this first intron which does not change the protein sequence results in a greater abundance of the unspliced form of the transcript than the spliced form. The full-length nature of the spliced transcript is not known. [provided by RefSeq Aug 2011]

Gene Symbol:   LAMB2
Gene Name:   laminin, beta 2 (laminin S)
Aliases:   LAMS
RefSeq:   NC_000003.11 NG_008094.1 NT_022517.18
Ensembl:   ENSG00000172037
Entrez:   3913
UniGene:   Hs.439726
Chromosome Mapping:   3p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.300000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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