Gene:  KDR, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Design and Validation of Real-Time PCR Primers
Pathway Curation
Control and Reference Assays for Real-Time PCR

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor known as kinase insert domain receptor is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation survival migration tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors including Rab GTPase P2Y purine nucleotide receptor integrin alphaVbeta3 T-cell protein tyrosine phosphatase etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq May 2009]

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PrimePCR™ SYBR® Green Assay: KDR, Human
kinase insert domain receptor (a type III receptor tyrosine kinase)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044206
Info:   Same primer pair as used in probe assay qHsaCEP0051468
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PrimePCR™ SYBR® Green Assay: KDR, Human
kinase insert domain receptor (a type III receptor tyrosine kinase)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0006310
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PrimePCR™ Probe Assay: KDR, Human
kinase insert domain receptor (a type III receptor tyrosine kinase)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051468
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PrimePCR™ ddPCR™ Copy Number Assay: KDR, Human
kinase insert domain receptor (a type III receptor tyrosine kinase)

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500417
Info:   66nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:KDR p.T1336T, Human
kinase insert domain receptor (a type III receptor tyrosine kinase)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502338
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:KDR WT for p.T1336T, Human
kinase insert domain receptor (a type III receptor tyrosine kinase)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502339
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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