PrimePCR™ SYBR® Green Assay: KDR, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0006310
Assay Design:   Intron-spanning
Chromosome Location:   4:55946255-55948711question
Amplicon Length:   140
Splice Variants Targeted:   ENST00000263923

Gene Information

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor known as kinase insert domain receptor is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation survival migration tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors including Rab GTPase P2Y purine nucleotide receptor integrin alphaVbeta3 T-cell protein tyrosine phosphatase etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq May 2009]

Gene Symbol:   KDR
Gene Name:   kinase insert domain receptor (a type III receptor tyrosine kinase)
Aliases:   CD309, FLK1, VEGFR, VEGFR2
RefSeq:   NC_000004.11 NG_012004.1 NT_022853.15
Ensembl:   ENSG00000128052
Entrez:   3791
UniGene:   Hs.479756
Chromosome Mapping:   4q11-q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 34.670000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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