Gene:  HRAS, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene belongs to the Ras oncogene family whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome a disease characterized by increased growth at the prenatal stage growth deficiency at the postnatal stage predisposition to tumor formation mental retardation skin and musculoskeletal abnormalities distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers including bladder cancer follicular thyroid cancer and oral squamous cell carcinoma. Multiple transcript variants which encode different isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: HRAS, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0023908
Info:   Same primer pair as used in probe assay qHsaCEP0033892
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PrimePCR™ Probe Assay: HRAS, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0033892
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PrimePCR™ ddPCR™ Copy Number Assay: HRAS, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000492
Info:   67nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: HRAS p.G12V, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000025
Info:   65nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: HRAS WT for p.G12V, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000026
Info:   65nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: HRAS p.Q61L, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500556
Info:   65nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: HRAS WT for p.Q61L, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500557
Info:   65nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: HRAS p.G12D, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500566
Info:   63nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: HRAS WT for p.G12D, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500567
Info:   63nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: HRAS p.G12S, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500568
Info:   63nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: HRAS WT for p.G12S, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500569
Info:   63nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: HRAS p.Q61R, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500576
Info:   65nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: HRAS WT for p.Q61R, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500577
Info:   65nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:HRAS p.G13D, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500668
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:HRAS WT for p.G13D, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500669
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:HRAS p.G12R, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500804
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:HRAS WT for p.G12R, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500805
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:HRAS p.G12C, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501880
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:HRAS WT for p.G12C, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501881
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:HRAS p.H27H, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502054
Info:   113nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:HRAS WT for p.H27H, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502055
Info:   113nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:HRAS p.E62G, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502472
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:HRAS WT for p.E62G, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502473
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:HRAS p.G13C, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502744
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:HRAS WT for p.G13C, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502745
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:HRAS p.Q61H, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502816
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:HRAS WT for p.Q61H, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502817
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:HRAS p.G12A, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502870
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:HRAS WT for p.G12A, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502871
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:HRAS p.Q61H, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503332
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:HRAS WT for p.Q61H, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503333
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:HRAS p.Q61K, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503784
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:HRAS WT for p.Q61K, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503785
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:HRAS p.G13V, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504008
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:HRAS WT for p.G13V, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504009
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:HRAS p.G13S, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504336
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:HRAS WT for p.G13S, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504337
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:HRAS p.G13R, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505560
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:HRAS WT for p.G13R, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505561
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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