Gene:  HRAS, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene belongs to the Ras oncogene family whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome a disease characterized by increased growth at the prenatal stage growth deficiency at the postnatal stage predisposition to tumor formation mental retardation skin and musculoskeletal abnormalities distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers including bladder cancer follicular thyroid cancer and oral squamous cell carcinoma. Multiple transcript variants which encode different isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: HRAS, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0023908
Info:   Same primer pair as used in probe assay qHsaCEP0033892
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PrimePCR™ Probe Assay: HRAS, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0033892
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PrimePCR™ ddPCR™ Copy Number Assay: HRAS, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000492
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: HRAS p.G12V, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000025
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: HRAS WT for p.G12V, Human
v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000026
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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