This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: HRAS, Human
PrimePCR™ Template for Probe Assay: HRAS, Human
This gene belongs to the Ras oncogene family whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome a disease characterized by increased growth at the prenatal stage growth deficiency at the postnatal stage predisposition to tumor formation mental retardation skin and musculoskeletal abnormalities distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers including bladder cancer follicular thyroid cancer and oral squamous cell carcinoma. Multiple transcript variants which encode different isoforms have been identified for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.