Gene:  HLA-F, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains this molecule is localized in the endoplasmic reticulum and Golgi apparatus with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site resulting in a shorter cytoplasmic domain. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: HLA-F, Human
major histocompatibility complex, class I, F

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0037356
Info:   Same primer pair as used in probe assay qHsaCEP0040079
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PrimePCR™ Probe Assay: HLA-F, Human
major histocompatibility complex, class I, F

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0040079
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: HLA-F, Human
major histocompatibility complex, class I, F

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5188492
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0037356; exonic
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PrimePCR™ ddPCR™ Expression Probe Assay: HLA-F, Human
major histocompatibility complex, class I, F

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5190675
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0040079; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: HLA-F, Human
major histocompatibility complex, class I, F

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5190676
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0040079; exonic
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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