PrimePCR™ SYBR® Green Assay: HLA-F, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040079

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HLA-F, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HLA-F, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037356
Assay Design:   exonic
Chromosome Location:   6:29691603-29691952question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000431367 ENST00000547791 ENST00000547167 ENST00000262032 ENST00000376861 ENST00000414333 ENST00000259951 ENST00000434407 ENST00000334668 ENST00000440587 ENST00000422711 ENST00000447400 ENST00000428296 ENST00000420067 ENST00000549067 ENST00000551832 ENST00000522452 ENST00000523522 ENST00000340042 ENST00000422439 ENST00000414958 ENST00000376844 ENST00000441200 ENST00000342322 ENST00000420414 ENST00000383516 ENST00000383515 ENST00000551026 ENST00000552082 ENST00000383626 ENST00000418017 ENST

Gene Information

This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains this molecule is localized in the endoplasmic reticulum and Golgi apparatus with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site resulting in a shorter cytoplasmic domain. [provided by RefSeq Jul 2008]

Gene Symbol:   HLA-F
Gene Name:   major histocompatibility complex, class I, F
Aliases:   CDA12, HLA-5.4, HLA-CDA12, HLAF
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1 NG_012009.1
Ensembl:   ENSG00000204642
Entrez:   3134
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.750000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download