Gene:  DAG1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein.[provided by RefSeq Apr 2010]

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PrimePCR™ SYBR® Green Assay: DAG1, Human
dystroglycan 1 (dystrophin-associated glycoprotein 1)

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0005494
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: DAG1, Human
dystroglycan 1 (dystrophin-associated glycoprotein 1)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0046978
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054239
List Price:    $125.00
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Add to Custom Plate
PrimePCR™ Probe Assay: DAG1, Human
dystroglycan 1 (dystrophin-associated glycoprotein 1)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0054239
List Price:    $355.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: DAG1, Human
dystroglycan 1 (dystrophin-associated glycoprotein 1)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5003377
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0005494; Exonic
List Price:    $120.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: DAG1, Human
dystroglycan 1 (dystrophin-associated glycoprotein 1)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5011356
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0046978; exonic
List Price:    $120.00
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PrimePCR™ ddPCR™ Expression Probe Assay: DAG1, Human
dystroglycan 1 (dystrophin-associated glycoprotein 1)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5041022
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0054239; exonic
 
Fluorophore:   FAM
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression Probe Assay: DAG1, Human
dystroglycan 1 (dystrophin-associated glycoprotein 1)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5041023
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0054239; exonic
 
Fluorophore:   HEX
List Price:    $180.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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