PrimePCR™ Probe Assay: DAG1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DAG1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DAG1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054239
Assay Design:   exonic
Chromosome Location:   3:49548089-49548186question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000566125 ENST00000421560 ENST00000308775 ENST00000418588 ENST00000431960 ENST00000435508 ENST00000452060 ENST00000428779 ENST00000515359 ENST00000545947 ENST00000541308 ENST00000539901 ENST00000538711 ENST00000373091 ENST00000382100 ENST00000382099 ENST00000317216 ENST00000512784 ENST00000509242 ENST00000556177 ENST00000380887 ENST00000261532 ENST00000444918 ENST00000339788 ENST00000320816 ENST00000509337 ENST00000545653

Gene Information

Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein.[provided by RefSeq Apr 2010]

Gene Symbol:   DAG1
Gene Name:   dystroglycan 1 (dystrophin-associated glycoprotein 1)
Aliases:   156DAG, A3a, AGRNR, DAG
RefSeq:   NC_000003.11 NG_013230.2 NT_022517.18
Ensembl:   ENSG00000173402
Entrez:   1605
Chromosome Mapping:   3p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.620000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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