Gene:  CDKN2A, Human

print
PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

Why PrimePCR?

PrimePCR
Lookup Tool
Design and Validation of Real-Time PCR Primers
Pathway Curation
Control and Reference Assays for Real-Time PCR

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with and sequester MDM1 a protein responsible for the degradation of p53. In spite of the structural and functional differences the CDK inhibitor isoforms and the ARF product encoded by this gene through the regulatory roles of CDK4 and p53 in cell cycle G1 progression share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors and is known to be an important tumor suppressor gene. [provided by RefSeq Jul 2008]

Select a product type :   All   qPCR   ddPCR
PrimePCR™ SYBR® Green Assay: CDKN2A, Human
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0023006
List Price:   
Your Price:   Log In
Quantity:  
 
Add to Custom Plate
PrimePCR™ SYBR® Green Assay: CDKN2A, Human
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0056722
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057827
List Price:   
Your Price:   Log In
Quantity:  
 
Add to Custom Plate
PrimePCR™ Probe Assay: CDKN2A, Human
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0057827
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Copy Number Assay: CDKN2A, Human
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000581
Info:   66nt amplicon; exonic
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay: CDKN2A p.R80*, Human
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500564
Info:   137nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay: CDKN2A WT for p.R80*, Human
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500565
Info:   137nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:CDKN2A p.P48L, Human
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501712
Info:   59nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:CDKN2A WT for p.P48L, Human
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501713
Info:   59nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:CDKN2A p.R58*, Human
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502016
Info:   137nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:CDKN2A WT for p.R58*, Human
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502017
Info:   137nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:CDKN2A p.A57V, Human
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502288
Info:   137nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:CDKN2A WT for p.A57V, Human
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502289
Info:   137nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:CDKN2A p.M52K, Human
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504314
Info:   64nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:CDKN2A WT for p.M52K, Human
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504315
Info:   64nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:CDKN2A p.A57P, Human
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505488
Info:   137nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:CDKN2A WT for p.A57P, Human
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505489
Info:   137nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:CDKN2A p.E61*, Human
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505520
Info:   137nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:CDKN2A WT for p.E61*, Human
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505521
Info:   137nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:CDKN2A p.D74Y, Human
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505996
Info:   137nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:CDKN2A WT for p.D74Y, Human
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505997
Info:   137nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.