This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: CDKN2A, Human
PrimePCR™ Template for SYBR® Green Assay: CDKN2A, Human
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with and sequester MDM1 a protein responsible for the degradation of p53. In spite of the structural and functional differences the CDK inhibitor isoforms and the ARF product encoded by this gene through the regulatory roles of CDK4 and p53 in cell cycle G1 progression share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors and is known to be an important tumor suppressor gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.