PrimePCR™ Probe Assay: APOBEC3A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: APOBEC3A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: APOBEC3A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040745
Assay Design:   exonic
Chromosome Location:   22:39358615-39358755question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000319921 ENST00000552744 ENST00000457153 ENST00000261205 ENST00000393240 ENST00000249116 ENST00000402255 ENST00000491143 ENST00000262095 ENST00000216218 ENST00000411695 ENST00000551589 ENST00000372592 ENST00000308700 ENST00000404699 ENST00000291294 ENST00000261523 ENST00000376351 ENST00000589078 ENST00000262623 ENST00000261980 ENST00000360319 ENST00000369850 ENST00000344736 ENST00000422373 ENST00000256062 ENST00000551659 ENST00000552618 ENST00000539277 ENST00000381224 ENST00000307808 ENST

Gene Information

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster thought to result from gene duplication on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene lacks the zinc binding activity of other family members. The protein plays a role in immunity by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines which leads to DNA degradation. However other mechanisms are also thought to be involved as anti-viral effect is not dependent on deaminase activity. One allele of this gene results from the deletion of approximately 29.5 kb of sequence between this gene APOBEC3A and the adjacent gene APOBEC3B. The breakpoints of the deletion are within the two genes so the deletion allele is predicted to have the promoter and coding region of APOBEC3A but the 3' UTR of APOBEC3B. [provided by RefSeq Jul 2010]

Gene Symbol:   APOBEC3A
Gene Name:   apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A
Aliases:   ARP3, PHRBN, bK150C2.1
RefSeq:   NC_000022.10 NT_011520.12 NW_003315972.1
Ensembl:   ENSG00000128383
Entrez:   200315
Chromosome Mapping:   22q13.1-q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.994900
y-intercept 36.560000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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