PrimePCR™ Probe Assay: Rpl13, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Rpl13, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Rpl13, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0028633
Assay Design:   intron_spanning
Chromosome Location:   19:66624276-66625368question
Amplicon Length:   194
Splice Variants Targeted:   ENSRNOT00000020635 ENSRNOT00000032780 ENSRNOT00000040069 ENSRNOT00000001995 ENSRNOT00000016623 ENSRNOT00000031005 ENSRNOT00000006492 ENSRNOT00000024885 ENSRNOT00000064613 ENSRNOT00000004532 ENSRNOT00000041303

Gene Information

a 60S ribosomal subunit protein [RGD, Feb 2006]

Gene Symbol:   Rpl13
Gene Name:   60S ribosomal protein L13
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000015335
Entrez:   81765
Chromosome Mapping:   19q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.930000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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