PrimePCR™ Probe Assay: Gpr68, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0032795
Assay Design:   exonic
Chromosome Location:   6:134532421-134532553question
Amplicon Length:   103
Splice Variants Targeted:   ENSRNOT00000013675 ENSRNOT00000005575 ENSRNOT00000048141 ENSRNOT00000038964 ENSRNOT00000073530 ENSRNOT00000074142

Gene Information

Description Not Available

Gene Symbol:   Gpr68
Gene Name:   Ovarian cancer G-protein coupled receptor 1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000049237
Entrez:   314386
Chromosome Mapping:   6q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.420000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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