Gene:  YWHAE, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases RAF1 and IRS1 proteins suggesting its role in diverse biochemical activities related to signal transduction such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants one protein-coding and the other non-protein-coding have been found for this gene. [provided by RefSeq Aug 2008]

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PrimePCR™ SYBR® Green Assay: YWHAE, Human
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043256
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050518
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PrimePCR™ SYBR® Green Assay: YWHAE, Human
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0022193
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PrimePCR™ Probe Assay: YWHAE, Human
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050518
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PrimePCR™ ddPCR™ Copy Number Assay: YWHAE, Human
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000551
Info:   69nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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