Gene:  WT1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern with biallelic and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq Oct 2010]

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PrimePCR™ SYBR® Green Assay: WT1, Human
Wilms tumor 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0014203
Info:   Same primer pair as used in probe assay qHsaCIP0029257
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PrimePCR™ Probe Assay: WT1, Human
Wilms tumor 1

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0029257
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PrimePCR™ ddPCR™ Copy Number Assay: WT1, Human
Wilms tumor 1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500489
Info:   67nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:WT1 p.R301*, Human
Wilms tumor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501632
Info:   64nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:WT1 WT for p.R301*, Human
Wilms tumor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501633
Info:   64nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:WT1 p.C282R, Human
Wilms tumor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501770
Info:   64nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:WT1 WT for p.C282R, Human
Wilms tumor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501771
Info:   64nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:WT1 p.D396N, Human
Wilms tumor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502082
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:WT1 WT for p.D396N, Human
Wilms tumor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502083
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:WT1 p.R394P, Human
Wilms tumor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502874
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:WT1 WT for p.R394P, Human
Wilms tumor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502875
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:WT1 p.R394Q, Human
Wilms tumor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503186
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:WT1 WT for p.R394Q, Human
Wilms tumor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503187
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:WT1 p.R394W, Human
Wilms tumor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505522
Info:   80nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:WT1 WT for p.R394W, Human
Wilms tumor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505523
Info:   80nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:WT1 p.S313*, Human
Wilms tumor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505630
Info:   76nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:WT1 WT for p.S313*, Human
Wilms tumor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505631
Info:   76nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:WT1 p.R390*, Human
Wilms tumor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505682
Info:   64nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:WT1 WT for p.R390*, Human
Wilms tumor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505683
Info:   64nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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