Gene:  VHL, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B elongin C and cullin-2 and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF) which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: VHL, Human
von Hippel-Lindau tumor suppressor

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0036545
Info:   Same primer pair as used in probe assay qHsaCEP0039269
List Price:    $125.00
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PrimePCR™ Probe Assay: VHL, Human
von Hippel-Lindau tumor suppressor

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0039269
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: VHL, Human
von Hippel-Lindau tumor suppressor

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5005760
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0036545; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Copy Number Assay: VHL, Human
von Hippel-Lindau tumor suppressor

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000521
Info:   FAM; 69nt amplicon; crosses exon-intron junction; FAM version of VHL (dHsaCP2506370)
 
Fluorophore:   FAM
List Price:    $230.00
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PrimePCR™ ddPCR™ Copy Number Assay: VHL, Human
von Hippel-Lindau tumor suppressor

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506370
Info:   HEX; 69nt amplicon; crosses exon-intron junction; HEX version of VHL (dHsaCP1000521)
 
Fluorophore:   HEX
List Price:    $230.00
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PrimePCR™ ddPCR™ Expression Probe Assay: VHL, Human
von Hippel-Lindau tumor suppressor

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5029832
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0039269; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: VHL, Human
von Hippel-Lindau tumor suppressor

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5029833
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0039269; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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PrimePCR™ ddPCR™ Mutation Assay:VHL p.S183*, Human
von Hippel-Lindau tumor suppressor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500770
Info:   76nt amplicon; exonic; in silico
 
Fluorophore:   FAM
List Price:    $315.00
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PrimePCR™ ddPCR™ Mutation Assay:VHL WT for p.S183*, Human
von Hippel-Lindau tumor suppressor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500771
Info:   76nt amplicon; exonic; in silico
 
Fluorophore:   HEX
List Price:    $315.00
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PrimePCR™ ddPCR™ Mutation Assay:VHL p.S80N, Human
von Hippel-Lindau tumor suppressor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502836
Info:   64nt amplicon; exonic; in silico
 
Fluorophore:   FAM
List Price:    $315.00
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PrimePCR™ ddPCR™ Mutation Assay:VHL WT for p.S80N, Human
von Hippel-Lindau tumor suppressor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502837
Info:   64nt amplicon; exonic; in silico
 
Fluorophore:   HEX
List Price:    $315.00
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PrimePCR™ ddPCR™ Mutation Assay:VHL p.R161*, Human
von Hippel-Lindau tumor suppressor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503094
Info:   65nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
List Price:    $315.00
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PrimePCR™ ddPCR™ Mutation Assay:VHL WT for p.R161*, Human
von Hippel-Lindau tumor suppressor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503095
Info:   65nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
List Price:    $315.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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