Gene:  Vegfa, Rat

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Design and Validation of Real-Time PCR Primers
Pathway Curation
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Control and Reference Assays for Real-Time PCR

This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. The expression of some isoforms derived from AUG start codon is affected by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2013]

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PrimePCR™ SYBR® Green Assay: Vegfa, Rat
Vascular endothelial growth factor A

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qRnoCED0002159
List Price:    $125.00
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PrimePCR™ Probe Assay: Vegfa, Rat
Vascular endothelial growth factor A

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qRnoCEP0023990
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: Vegfa, Rat
Vascular endothelial growth factor A

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dRnoEG5125452
Info:   EG; Same primer pair as used in qPCR assay qRnoCED0002159; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: Vegfa, Rat
Vascular endothelial growth factor A

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dRnoCPE5146882
Info:   FAM; Same primer pair and probe as used in qPCR assay qRnoCEP0023990; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: Vegfa, Rat
Vascular endothelial growth factor A

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dRnoCPE5146883
Info:   HEX; Same primer pair and probe as used in qPCR assay qRnoCEP0023990; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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