Gene:  UBE3A, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes an E3 ubiquitin-protein ligase part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome characterized by severe motor and intellectual retardation ataxia hypotonia epilepsy absence of speech and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18 resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described but their full length nature has not been determined. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: UBE3A, Human
ubiquitin protein ligase E3A

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0016100
Info:   Same primer pair as used in probe assay qHsaCIP0030373
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PrimePCR™ Probe Assay: UBE3A, Human
ubiquitin protein ligase E3A

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0030373
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PrimePCR™ ddPCR™ Copy Number Assay: UBE3A, Human
ubiquitin protein ligase E3A

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000312
 
Fluorophore:   FAM
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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