Gene:  Tyms, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes an enzyme that catalyzes the methylation of deoxyuridylate to deoxythymidylate using 510-methylenetetrahydrofolate as a cofactor. This function maintains the thymidine-5-prime monophosphate concentration critical for DNA replication and repair. The encoded enzyme is a target for cancer chemotherapeutic agents. The majority of transcripts for this gene lack a 3' UTR (PMID: 3022294 3444407). The stop codon in these transcripts is UAA compared to the UAG found in the genome and longer transcripts as the polyA site is located within the stop codon (PMID: 3444407 2157203). A related pseudogene has been identified on chromosome 10. [provided by RefSeq Mar 2010]

PrimePCR™ SYBR® Green Assay: Tyms, Mouse
Thymidylate synthase

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qMmuCED0046162
Info:   Same primer pair as used in probe assay qMmuCEP0054588
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PrimePCR™ SYBR® Green Assay: Tyms, Mouse
Thymidylate synthase

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qMmuCID0005615
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: Tyms, Mouse
Thymidylate synthase

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qMmuCEP0054588
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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