Gene:  TP53, Human

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This gene encodes tumor protein p53 which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest apoptosis senescence DNA repair or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation DNA-binding and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms which can regulate p53 transcriptional activity. [provided by RefSeq Jul 2008]

Select a product type :   All   qPCR   ddPCR
PrimePCR™ SYBR® Green Assay: TP53, Human
tumor protein p53

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0045022
Info:   Same primer pair as used in probe assay qHsaCEP0052284
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PrimePCR™ SYBR® Green Assay: TP53, Human
tumor protein p53

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0013658
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: TP53, Human
tumor protein p53

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0052284
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PrimePCR™ ddPCR™ Copy Number Assay: TP53, Human
tumor protein p53

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000586
Info:   65nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.R249S, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000087
Info:   65nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.R249S, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000088
Info:   65nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.Y163C, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000099
Info:   79nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.Y163C, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000100
Info:   79nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.R175H, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000105
Info:   65nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.R175H, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000106
Info:   65nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.R248W, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000107
Info:   65nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.R248W, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000108
Info:   65nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.R273H, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000109
Info:   65nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.R273H, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000110
Info:   65nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.R196*, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000121
Info:   65nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.R196*, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000122
Info:   65nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.H179R, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000125
Info:   81nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.H179R, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000126
Info:   81nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.R248Q, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000127
Info:   62nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.R248Q, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000128
Info:   62nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.V157F, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000129
Info:   62nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.V157F, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000130
Info:   62nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.R248L, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500502
Info:   62nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.R248L, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500503
Info:   62nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.R273L, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500504
Info:   65nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.R273L, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500505
Info:   65nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.V173M, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500514
Info:   64nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.V173M, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500515
Info:   64nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.R175L, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500518
Info:   65nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.R175L, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500519
Info:   65nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.H179Y, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500520
Info:   81nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.H179Y, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500521
Info:   81nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.R158H, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500522
Info:   62nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.R158H, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500523
Info:   62nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.Y220C, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500536
Info:   64nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.Y220C, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500537
Info:   64nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.R273C, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500538
Info:   65nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.R273C, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500539
Info:   65nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.H179Y, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500540
Info:   81nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.H179Y, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500541
Info:   81nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.G245D, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500542
Info:   65nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.G245D, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500543
Info:   65nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.R306*, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500552
Info:   65nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.R306*, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500553
Info:   65nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.G245V, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500554
Info:   70nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.G245V, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500555
Info:   70nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: TP53 p.P151S, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500570
Info:   65nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: TP53 WT for p.P151S, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500571
Info:   65nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:TP53 p.Q144L, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500600
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:TP53 WT for p.Q144L, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500601
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:TP53 p.T125T, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500626
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:TP53 WT for p.T125T, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500627
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:TP53 p.C238Y, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500628
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:TP53 WT for p.C238Y, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500629
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:TP53 p.G244G, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500644
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:TP53 WT for p.G244G, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500645
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:TP53 p.G266R, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500648
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:TP53 WT for p.G266R, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500649
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:TP53 p.P222L, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500656
Info:   66nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:TP53 WT for p.P222L, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500657
Info:   66nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:TP53 p.K164M, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500678
Info:   62nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:TP53 WT for p.K164M, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500679
Info:   62nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:TP53 p.E339*, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500684
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:TP53 WT for p.E339*, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500685
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:TP53 p.L194R, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500686
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:TP53 WT for p.L194R, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500687
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:TP53 p.R248P, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500692
Info:   62nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:TP53 WT for p.R248P, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500693
Info:   62nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:TP53 p.R273P, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500708
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:TP53 WT for p.R273P, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500709
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:TP53 p.H168Y, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500720
Info:   69nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:TP53 WT for p.H168Y, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500721
Info:   69nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:TP53 p.L265P, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500726
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:TP53 WT for p.L265P, Human
tumor protein p53

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500727
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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