Gene:  TOP2A, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a DNA topoisomerase an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation chromatid separation and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form alpha is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq Jul 2010]

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PrimePCR™ SYBR® Green Assay: TOP2A, Human
topoisomerase (DNA) II alpha 170kDa

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0057177
Info:   Same primer pair as used in probe assay qHsaCEP0058282
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PrimePCR™ SYBR® Green Assay: TOP2A, Human
topoisomerase (DNA) II alpha 170kDa

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0006279
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: TOP2A, Human
topoisomerase (DNA) II alpha 170kDa

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0058282
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PrimePCR™ ddPCR™ Copy Number Assay: TOP2A, Human
topoisomerase (DNA) II alpha 170kDa

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000459
Info:   68nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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