Gene:  TGFBR2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. The encoded protein is a transmembrane protein that has a protein kinase domain forms a heterodimeric complex with another receptor protein and binds TGF-beta. This receptor/ligand complex phosphorylates proteins which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan Syndrome Loeys-Deitz Aortic Aneurysm Syndrome and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: TGFBR2, Human
transforming growth factor, beta receptor II (70/80kDa)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0016240
Info:   Same primer pair as used in probe assay qHsaCIP0030460
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PrimePCR™ Probe Assay: TGFBR2, Human
transforming growth factor, beta receptor II (70/80kDa)

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0030460
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PrimePCR™ ddPCR™ Copy Number Assay: TGFBR2, Human
transforming growth factor, beta receptor II (70/80kDa)

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000546
Info:   70nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:TGFBR2 p.D446N, Human
transforming growth factor, beta receptor II (70/80kDa)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502652
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:TGFBR2 WT for p.D446N, Human
transforming growth factor, beta receptor II (70/80kDa)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502653
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:TGFBR2 p.R528H, Human
transforming growth factor, beta receptor II (70/80kDa)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504442
Info:   73nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:TGFBR2 WT for p.R528H, Human
transforming growth factor, beta receptor II (70/80kDa)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504443
Info:   73nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:TGFBR2 p.R497*, Human
transforming growth factor, beta receptor II (70/80kDa)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505956
Info:   64nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:TGFBR2 WT for p.R497*, Human
transforming growth factor, beta receptor II (70/80kDa)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505957
Info:   64nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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