Gene:  TERT, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity encoded by this gene and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: TERT, Human
telomerase reverse transcriptase

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0009247
Info:   Same primer pair as used in probe assay qHsaCIP0027108
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PrimePCR™ Probe Assay: TERT, Human
telomerase reverse transcriptase

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0027108
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PrimePCR™ ddPCR™ Copy Number Assay: TERT, Human
telomerase reverse transcriptase

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000059
Info:   100nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Copy Number Assay: TERT, Human
telomerase reverse transcriptase

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000100
Info:   100nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Copy Number Assay: TERT, Human
telomerase reverse transcriptase

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500314
Info:   70nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Copy Number Assay: TERT, Human
telomerase reverse transcriptase

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500351
Info:   70nt amplicon; exonic
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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