Gene:  STAT4, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors STAT family members are phosphorylated by the receptor associated kinases and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq Aug 2011]

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PrimePCR™ SYBR® Green Assay: STAT4, Human
signal transducer and activator of transcription 4

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0018660
Info:   Same primer pair as used in probe assay qHsaCIP0031987
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PrimePCR™ Probe Assay: STAT4, Human
signal transducer and activator of transcription 4

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0031987
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PrimePCR™ ddPCR™ Mutation Assay:STAT4 p.R400*, Human
signal transducer and activator of transcription 4

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503304
Info:   97nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:STAT4 WT for p.R400*, Human
signal transducer and activator of transcription 4

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503305
Info:   97nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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