Gene:  SPTA1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton and functions in the determination of cell shape arrangement of transmembrane proteins and organization of organelles. It is a tetramer made up of alpha-beta dimers linked in a head-to-head arrangement. This gene is one member of a family of alpha-spectrin genes. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms weaker tetramer interactions than non-erythrocytic alpha spectrin which may increase the plasma membrane elasticity and deformability of red blood cells. Mutations in this gene result in a variety of hereditary red blood cell disorders including elliptocytosis type 2 pyropoikilocytosis and spherocytic hemolytic anemia. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: SPTA1, Human
spectrin, alpha, erythrocytic 1 (elliptocytosis 2)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0014690
Info:   Same primer pair as used in probe assay qHsaCIP0029512
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PrimePCR™ Probe Assay: SPTA1, Human
spectrin, alpha, erythrocytic 1 (elliptocytosis 2)

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0029512
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PrimePCR™ ddPCR™ Mutation Assay:SPTA1 p.R268*, Human
spectrin, alpha, erythrocytic 1 (elliptocytosis 2)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501030
Info:   70nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SPTA1 WT for p.R268*, Human
spectrin, alpha, erythrocytic 1 (elliptocytosis 2)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501031
Info:   70nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SPTA1 p.A2011V, Human
spectrin, alpha, erythrocytic 1 (elliptocytosis 2)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504690
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SPTA1 WT for p.A2011V, Human
spectrin, alpha, erythrocytic 1 (elliptocytosis 2)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504691
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SPTA1 p.N963N, Human
spectrin, alpha, erythrocytic 1 (elliptocytosis 2)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505884
Info:   67nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SPTA1 WT for p.N963N, Human
spectrin, alpha, erythrocytic 1 (elliptocytosis 2)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505885
Info:   67nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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