Gene:  SPO11, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Meiotic recombination and chromosome segregation require the formation of double-strand breaks (DSBs) in paired chromosome homologs. During meiosis in yeast a meiotic recombination protein is covalently-linked to the 5' end of DSBs and is essential for the formation of DSBs. The protein encoded by this gene is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene but the full-length nature of only two of them have been described. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: SPO11, Human
SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0057113
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0058218
List Price:    $130.00
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PrimePCR™ SYBR® Green Assay: SPO11, Human
SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0009522
List Price:    $130.00
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PrimePCR™ Probe Assay: SPO11, Human
SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0058218
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SPO11, Human
SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5013690
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0057113; exonic
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SPO11, Human
SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5016457
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0009522; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: SPO11, Human
SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5045690
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0058218; exonic
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: SPO11, Human
SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5045691
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0058218; exonic
 
Fluorophore:   HEX
List Price:    $195.00
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Quantity:  
 
    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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