Gene:  SMAD4, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer juvenile polyposis syndrome and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq Oct 2009]

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PrimePCR™ SYBR® Green Assay: SMAD4, Human
SMAD family member 4

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0015670
Info:   Same primer pair as used in probe assay qHsaCIP0030084
List Price:    $125.00
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PrimePCR™ Probe Assay: SMAD4, Human
SMAD family member 4

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0030084
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SMAD4, Human
SMAD family member 4

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5021011
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0015670; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Copy Number Assay: SMAD4, Human
SMAD family member 4

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500468
Info:   FAM; 70nt amplicon; exonic; FAM version of SMAD4 (dHsaCP2506563)
 
Fluorophore:   FAM
List Price:    $230.00
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PrimePCR™ ddPCR™ Copy Number Assay: SMAD4, Human
SMAD family member 4

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506563
Info:   HEX; 70nt amplicon; exonic; HEX version of SMAD4 (dHsaCP2500468)
 
Fluorophore:   HEX
List Price:    $230.00
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PrimePCR™ ddPCR™ Expression Probe Assay: SMAD4, Human
SMAD family member 4

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5053496
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0030084; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: SMAD4, Human
SMAD family member 4

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5053497
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0030084; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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PrimePCR™ ddPCR™ Mutation Assay:SMAD4 p.R361C, Human
SMAD family member 4

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505076
Info:   65nt amplicon; exonic; in silico
 
Fluorophore:   FAM
List Price:    $315.00
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PrimePCR™ ddPCR™ Mutation Assay:SMAD4 WT for p.R361C, Human
SMAD family member 4

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505077
Info:   65nt amplicon; exonic; in silico
 
Fluorophore:   HEX
List Price:    $315.00
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PrimePCR™ ddPCR™ Mutation Assay:SMAD4 p.R361H, Human
SMAD family member 4

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505388
Info:   65nt amplicon; exonic; in silico
 
Fluorophore:   FAM
List Price:    $315.00
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PrimePCR™ ddPCR™ Mutation Assay:SMAD4 WT for p.R361H, Human
SMAD family member 4

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505389
Info:   65nt amplicon; exonic; in silico
 
Fluorophore:   HEX
List Price:    $315.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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